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Image Search Results
Journal: Viruses
Article Title: Divergent Traits and Ligand-Binding Properties of the Cytomegalovirus CD48 Gene Family
doi: 10.3390/v12080813
Figure Lengend Snippet: Expression of vCD48s during OMCMV and SMCMV infections. Owl monkey kidney (OMK) cells (left panels) or HEL299 (right panels) cells were mock infected or infected with OMCMV or SMCMV at an moi of 1 in the absence (-) or presence of cycloheximide (CHX) or phosphonoacetic acid (PPA), as indicated in the Materials and Methods. Whole-cell RNAs were harvested at 13 hpi in the case of CHX samples and at 72 hpi for the rest of the samples, and subsequently reverse transcribed. PCRs were performed using primer sets specific for OMCMV genes A44 , A45 , IE1 , UL54 , and UL73 , SMCMV genes S30 , S31 , IE1 , UL54 , and UL73 , or GAPDH , as shown. Amplified products were separated on 1% agarose gels and visualized by RedSafe nucleic acid staining solution.
Article Snippet: Samples were precleared three times for 30 min using
Techniques: Expressing, Infection, Amplification, Staining